Milla Jovovich

Alice

Anna Bolt

Dr. Green

Joseph May

Dr. Blue

Robert Tannion

Dr. Brown

Jaymes Butler

Clarence

Stephen Billington

Mr. White

Fiona Glascott

Ms. Gold

Liz May Brice

Medic

Michaela Dicker

Red Queen

Oscar Pearce

Mr. Red

Indra Ové

Ms. Black

Michelle Rodriguez

Rain Ocampo

Eric Mabius

Matt Addison

James Purefoy

Spence Parks

Martin Crewes

Chad Kaplan

Colin Salmon

James

Pasquale Aleardi

J.D. Salinas

Heike Makatsch

Dr. Lisa Addison

Ryan McCluskey

Mr. Grey

Jason Isaacs

Dr. William Birkin (uncredited)